The IP3 (Inositol Triphosphate) ELISA Kit is a reliable and efficient tool for the accurate measurement of IP3 levels in a variety of sample types including cell lysates, tissue homogenates, and biological fluids. This kit offers high sensitivity and specificity, allowing for precise and reproducible results in a wide range of research applications.IP3 is a crucial second messenger molecule involved in signaling pathways that regulate various cellular processes including cell growth, differentiation, and metabolism.
Dysregulation of IP3 levels has been implicated in numerous diseases such as cancer, neurological disorders, and metabolic disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the IP3 ELISA Kit is a valuable tool for researchers seeking to investigate the role of IP3 in cellular signaling pathways and its potential implications in disease pathogenesis.
Product Name:
IP3 (Inositol Triphosphate) ELISA Kit (UNES00039)
SKU:
UNES00039
Target:
IP3 (Inositol Triphosphate) (UNES00039)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with IP3. During the reaction, IP3 in the sample or standard competes with a fixed amount of IP3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to IP3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IP3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
89-102
96-110
Average (%)
103
96
101
1:4
Range (%)
89-104
93-105
96-113
Average (%)
95
98
104
1:8
Range (%)
88-104
89-105
98-114
Average (%)
95
96
105
1:16
Range (%)
84-98
89-104
96-112
Average (%)
90
96
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
93
EDTA plasma (n=5)
88-98
93
Cell culture media (n=5)
85-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.4
130.4
426.2
58.4
132.3
424.5
Standard deviation
2.7
5.2
16.2
4.0
7.7
14.9
C V (%)
4.96
3.99
3.8
6.85
5.82
3.51
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of IP3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes IP3 in samples. No significant cross-reactivity or interference between IP3 and analogues was observed.
