Invertase Activity Assay Kit (BA0123)

SKU:

BA0123

Product Type:

Assay

Instrument:

Microplate Reader

Sample Type:

Biological, Environment (Soil)

Research Area:

Plant & Environmental Stress

Enzyme Activity

Description

Description

Invertase Activity Assay Kit

Invertase (b-fructofuranosidase, EC 3.2.1.26) is an enzyme that catalyzes the hydrolysis of sucrose to fructose and glucose. Invertases cleave at the O-C(fructose) bond, whereas a related enzyme sucrase (EC 3.2.1.48) cleaves at the O-C(glucose) bond. A wide range of microorganisms produce invertase and can, thus, utilize sucrose as a nutrient. Invertase assay finds wide applications in environmental (e.g. soil), agricultural and food (confectionery) industry.

Assay Genie's Invertase activity Kit provides a convenient and ultra-sensitive colorimetric and fluorimetric means to measure invertase activity. In the assay, invertase cleaves sucrose, resulting in the formation of fructose and glucose, which is determined by a colorimetric (570nm) or fluorimetric method (Ex/Em = 530/585nm). The assay is simple, sensitive, stable and high-throughput adaptable.

Applications

For quantitative determination of invertase/sucrase activity

Key Features

Safe. Non-radioactive assay.
Sensitive and accurate. As low as 0.007 U/L invertase activity can be quantified.
Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.
Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Invertase Activity Assay Kit Information:

Kit Includes:	10x Reaction Buffer: 12 mL (pH 4.5), 10x Sucrose: 1.5 mL, Assay Buffer: 10 mL, Enzyme Mix: 120 mL Glucose Standard: 1 mL Dye Reagent: 120 mL
Kit Requires:	Pipetting devices, centrifuge tubes, clear or black flat bottom 96-well plate.
Method of Detection:	OD570nm, or FL530/585nm
Detection Limit:	0.007 U/L
Samples:	Biological, environment (soil) etc
Species:	All
Protocol Length:	40 min
Size:	100 tests
Storage:	Store Reaction Buffer and Assay Buffer at 4°C and other reagents at - 20°C.
Shelf Life:	6 months