The INTS3 Polyclonal Antibody (PACO58801) is a valuable tool for researchers studying the INTS3 protein, a component of the Integrator complex involved in transcriptional regulation. This antibody, generated in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications.The INTS3 antibody specifically binds to the INTS3 protein, allowing for its detection and analysis in a variety of cell types. This makes it an ideal choice for studies in molecular biology, gene regulation, and RNA processing.
By understanding the function of INTS3, researchers can gain insights into its role in cellular processes and potentially uncover new therapeutic targets for diseases related to transcriptional dysregulation.Overall, the INTS3 Polyclonal Antibody is a reliable tool for researchers looking to investigate the functions of INTS3 and its implications in gene expression and transcriptional control. Its high specificity and sensitivity make it a valuable asset in the field of molecular biology and gene regulation research.
Antibody Name:
INTS3 Antibody (PACO58801)
Antibody SKU:
PACO58801
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Integrator complex subunit 3 protein (901-1043AA)
Immunofluorescence staining of Hela cells with PACO58801 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Component of the Integrator (INT) complex. The Integrator complex is involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes (Probable). Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the INT complex.
Synonyms:
Integrator complex subunit 3 (Int3) (SOSS complex subunit A) (Sensor of single-strand DNA complex subunit A) (SOSS-A) (Sensor of ssDNA subunit A), INTS3, C1orf193 C1orf60
UniProt Protein Function:
INTS3: Component of the Integrator complex. The Integrator complex is involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Belongs to the Integrator subunit 3 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Chromosomal Location of Human Ortholog: 1q21.3
Cellular Component: integrator complex; nucleus
Molecular Function:protein binding
Biological Process: DNA repair; mitotic cell cycle checkpoint; response to DNA damage stimulus; response to ionizing radiation; snRNA processing
NCBI Summary:
INTS3 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]). INTS3 is also a subunit of single-stranded DNA (ssDNA)-binding complexes involved in the maintenance of genome stability (Huang et al., 2009) [PubMed 19683501].[supplied by OMIM, Feb 2010]