The INTS11 Monoclonal Antibody (ABM71012) is a valuable tool for researchers studying the INTS11 protein, a key component of the integrator complex involved in RNA transcription and processing. This antibody, generated by hybridoma technology, specifically targets the INTS11 protein in human samples and is validated for use in various experimental applications, including immunofluorescence and flow cytometry.INTS11 plays a crucial role in the regulation of gene expression, making it an important target for research in areas such as developmental biology, cancer biology, and neurobiology.
By targeting INTS11, researchers can gain insights into the mechanisms underlying gene transcription and its dysregulation in diseases. This monoclonal antibody provides a reliable tool for detecting and localizing INTS11 in cells and tissues, facilitating detailed analysis of its functions in different biological contexts.
The Integrator complex contains at least 12 subunits and associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates the 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690). INTS11, or CPSF3L, is the catalytic subunit of the Integrator complex (Baillat et al., 2005 [PubMed 16239144]).
Purification Method:
Affinity purification
Gene ID:
54973
Clone Number:
ARC58804
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using INTS11 antibody (CAB22698) at 1:6000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
