The INTS10 Polyclonal Antibody (CAB6632) is a high-quality research tool designed for studying INTS10, a protein involved in various cellular processes, including RNA splicing and transcription. This antibody, generated in rabbits, exhibits strong reactivity with human samples and is validated for use in Western blot applications. INTS10, a subunit of the integrator complex, plays a key role in the proper functioning of RNA polymerase II transcription and RNA processing. Dysregulation of INTS10 has been implicated in diseases such as cancer and neurodevelopmental disorders.
By targeting INTS10 with this antibody, researchers can further investigate its function and potential contribution to disease pathology.This antibody is essential for researchers interested in unraveling the molecular mechanisms underlying gene expression and RNA metabolism. Its specificity and sensitivity make it an invaluable tool for studies aiming to elucidate the role of INTS10 in various biological processes, providing valuable insights for drug discovery and therapeutic development.
Product Name:
INTS10 Rabbit Polyclonal Antibody
SKU:
CAB6632
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 451-710 of human INTS10 (NP_060612.2).
INTS10 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]).
Purification Method:
Affinity purification
Gene ID:
55174
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using INTS10 antibody (CAB6632) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
