The INTS10 Polyclonal Antibody (CAB13419) is a valuable tool for researchers studying INTS10, a protein involved in the integrator complex that plays a crucial role in RNA polymerase II transcription. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in immunofluorescence and Western blot applications.INTS10 is a key component of the integrator complex, which functions in the 3'-end processing of small nuclear RNAs and plays a role in the regulation of gene expression. Research on INTS10 is important for understanding its role in gene transcription, RNA processing, and potentially its involvement in diseases related to aberrant gene expression.
The INTS10 Polyclonal Antibody enables researchers to detect and analyze INTS10 protein expression in various cell types, providing valuable insights into its function and potential implications in gene regulation. Its high specificity and reliability make it an essential tool for studies in molecular biology, gene expression regulation, and RNA processing pathways.
Product Name:
INTS10 Rabbit Polyclonal Antibody
SKU:
CAB13419
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 451-710 of human INTS10 (NP_060612.2).
INTS10 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]).
Purification Method:
Affinity purification
Gene ID:
55174
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using INTS10 antibody (CAB13419) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
