The INTS1 Polyclonal Antibody (PAC019164) is a valuable tool for researchers studying the INTS1 protein, an essential component of the integrator complex involved in RNA processing. This antibody is produced in rabbits and has been validated for use in Western blot applications, providing researchers with a reliable tool for detecting and analyzing INTS1 in various cell types.INTS1 is a key player in the regulation of gene expression and has been implicated in various cellular processes, including RNA splicing and transcription. Dysfunction of INTS1 has been associated with developmental disorders and cancer, highlighting the importance of studying its functions in different biological contexts.
By using the INTS1 Polyclonal Antibody, researchers can gain insights into the molecular mechanisms regulated by INTS1 and its potential role in disease pathogenesis.Overall, the INTS1 Polyclonal Antibody is a valuable resource for researchers interested in understanding the intricate mechanisms of RNA processing and gene regulation, with potential implications for the development of novel therapeutic strategies targeting INTS1-related pathways.
Antibody Name:
INTS1 Antibody (PACO19164)
Antibody SKU:
PACO19164
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:25-1:100
Species Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide of human INTS1
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using PACO19164(INTS1 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
INTS1 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit and mediates 3-prime end processing of small nuclear RNAs U1 and U2. RNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin.
Synonyms:
integrator complex subunit 1
UniProt Protein Function:
INTS1: Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Belongs to the Integrator subunit 1 family.Protein type: RNA processing; Membrane protein, integralChromosomal Location of Human Ortholog: 7p22.3Cellular Component: nuclear membrane; membrane; integral to membrane; integrator complexBiological Process: snRNA processing; negative regulation of caspase activity; inner cell mass cell proliferation
UniProt Protein Details:
NCBI Summary:
INTS1 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]).[supplied by OMIM, Mar 2008]
