The Interleukin-7 Polyclonal Antibody (CAB1650) is a valuable tool for researchers studying Interleukin-7 (IL-7), a cytokine that plays a crucial role in immune system development and function. This antibody, developed and validated for use in Western blotting, is raised in rabbits and exhibits high specificity for detecting IL-7 in human samples.IL-7 is known for its essential role in T cell development, survival, and homeostasis, making it a key player in immune responses and autoimmune disorders. By targeting IL-7 with this polyclonal antibody, researchers can gain insights into how this cytokine functions in various cellular contexts, providing valuable information for immunology, cancer, and autoimmune disease research.
With its ability to specifically recognize and bind to IL-7, the Interleukin-7 Polyclonal Antibody (CAB1650) offers a reliable tool for investigating the role of this cytokine in health and disease. Its application in Western blot assays enables accurate detection and analysis of IL-7 levels, making it a valuable asset for researchers seeking to understand the complex mechanisms of immune regulation.
Product Name:
IL7 Rabbit Polyclonal Antibody
SKU:
CAB1650
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 26-177 of human IL7 (NP_000871.1).
The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL7 is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. IL7 plays an essential role in lymphoid cell survival, and in the maintenance of naive and memory T cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can be a potent inducer of proinflammatory cytokines and chemokines which may defend against the infection, but may also mediate destructive lung injury. Elevated serum IL7 levels, together with several other circulating cytokines and chemokines, has been found to be associated with the severity of Coronavirus Disease 19 (COVID-19).
Purification Method:
Affinity purification
Gene ID:
3574
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using IL7 antibody (CAB1650) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
