null

IL4 Rabbit Polyclonal Antibody (CAB5649)

SKU:
CAB5649
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

IL4 Rabbit Polyclonal Antibody (CAB5649)

The Interleukin-4 Polyclonal Antibody (CAB5649) is a valuable tool for researchers studying interleukin-4 (IL-4), a key cytokine involved in immune response regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.IL-4 is known for its role in promoting Th2 cell differentiation, B cell proliferation, and antibody class switching. It is also involved in the regulation of allergic responses and inflammation. The Interleukin-4 Polyclonal Antibody binds specifically to IL-4, allowing for accurate detection and analysis in a variety of cell types.

Research on IL-4 is crucial for understanding its involvement in various diseases, including asthma, allergy, and autoimmune disorders. By targeting IL-4, researchers aim to develop new therapies that modulate immune responses and treat these conditions effectively.Overall, the Interleukin-4 Polyclonal Antibody is a reliable tool for investigating the role of IL-4 in immune regulation and disease pathogenesis, making it a valuable asset for immunologists and cancer researchers alike.

Product Name:IL4 Rabbit Polyclonal Antibody
SKU:CAB5649
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-153 of human IL4 (NP_000580.1).
Sequence:HKCD ITLQ EIIK TLNS LTEQ KTLC TELT VTDI FAAS KNTT EKET FCRA ATVL RQFY SHHE KDTR CLGA TAQQ FHRH KQLI RFLK RLDR NLWG LAGL NSCP VKEA NQST LENF LERL KTIM REKY SKCS S
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IHC-P,1:50 - 1:100 IF/ICC,1:50 - 1:200
Synonyms:BSF1; IL-4; BCGF1; BSF-1; BCGF-1; IL4
Positive Sample:293T transfected with IL4 protein
Conjugate:Unconjugated
Cellular Localization:Secreted.
Calculated MW:17kDa
Observed MW:20kDa

The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. IL4 is considered an important cytokine for tissue repair, counterbalancing the effects of proinflammatory type 1 cytokines, however, it also promotes allergic airway inflammation. Moreover, IL-4, a type 2 cytokine, mediates and regulates a variety of human host responses such as allergic, anti-parasitic, wound healing, and acute inflammation. This cytokine has been reported to promote resolution of neutrophil-mediated acute lung injury. In an allergic response, IL-4 has an essential role in the production of allergen-specific immunoglobin (Ig) E. This pro-inflammatory cytokine has been observed to be increased in COVID-19 (Coronavirus disease 2019) patients, but is not necessarily associated with severe COVID-19 pathology. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Purification Method:Affinity purification
Gene ID:3565
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and 293T cells transfected with IL4 using IL4 Rabbit pAb(CAB5649) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 0.5s.

Related Products