The Interleukin-2 Polyclonal Antibody (CAB0302) is a valuable tool for researchers studying interleukin-2 (IL-2), a key cytokine involved in immune regulation and cell growth. Raised in rabbits, this antibody is specifically designed for use in Western blot applications and is highly reactive with human samples. By binding to the IL-2 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential tool for studies in immunology and cancer research.IL-2 plays a vital role in the regulation of immune responses and the activation of T cells, making it a crucial target for research into immune disorders, such as autoimmune diseases and cancer.
By understanding the function and activity of IL-2, researchers can develop innovative therapies that target immune cells and enhance immune responses in various disease contexts. The Interleukin-2 Polyclonal Antibody (CAB0302) provides researchers with a reliable tool to explore the diverse functions of IL-2 and its implications for immunotherapy and disease treatment.
Product Name:
IL2 Rabbit Polyclonal Antibody
SKU:
CAB0302
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 21-153 of human IL2 (NP_000577.2).
Jurkat treated by TPA,A23187and Brefeldin A,Recombinant Human IL2 Protein
Conjugate:
Unconjugated
Cellular Localization:
Secreted.
Calculated MW:
18kDa
Observed MW:
15-20kDa
This gene is a member of the interleukin 2 (IL2) cytokine subfamily which includes IL4, IL7, IL9, IL15, IL21, erythropoietin, and thrombopoietin. The protein encoded by this gene is a secreted cytokine produced by activated CD4+ and CD8+ T lymphocytes, that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2R) is a heterotrimeric protein complex whose gamma chain is also shared by IL4 and IL7. The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
Purification Method:
Affinity purification
Gene ID:
3558
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using IL2 Rabbit pAb (CAB0302) at 1:1000 dilution.Jurkat cells were treated by TPA (40 nM),A23187 (2μM) and Brefeldin A (300 ng / ml) for 0-24hoursSecondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
