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Anti-IL-10 Antibody (CAB2171)

SKU:
CAB2171
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • Cell Biology Antibodies 8 Anti-IL-10 Antibody CAB2171
  • Cell Biology Antibodies 8 Anti-IL-10 Antibody CAB2171
$167 - $503

Description

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Anti-IL-10 Antibody (CAB2171)

The Interleukin-10 Polyclonal Antibody (CAB2171) is a vital tool for researchers studying interleukin-10, a key anti-inflammatory cytokine with immunoregulatory functions. This antibody is produced in rabbits and has high reactivity with human samples, making it suitable for use in a variety of research applications including Western blotting.Interleukin-10 is known for its role in dampening immune responses and promoting immune tolerance, making it a critical mediator in various disease states such as infections, autoimmunity, and inflammation.

By targeting interleukin-10, researchers can gain insights into the mechanisms underlying these diseases and potentially develop new therapeutic strategies.This polyclonal antibody specifically binds to interleukin-10, allowing for the detection and analysis of this cytokine in different cell types and biological samples. Its versatility and effectiveness make it an essential tool for studying the complex interactions of interleukin-10 in the immune system and its potential implications for disease pathogenesis.

Antibody Name:IL10 Rabbit Polyclonal Antibody
Antibody SKU:CAB2171
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 19-178 of human IL10 (NP_000563.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 19-178 of human IL10 (NP_000563.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SPGQ GTQS ENSC THFP GNLP NMLR DLRD AFSR VKTF FQMK DQLD NLLL KESL LEDF KGYL GCQA LSEM IQFY LEEV MPQA ENQD PDIK AHVN SLGE NLKT LRLR LRRC HRFL PCEN KSKA VEQV KNAF NKLQ EKGI YKAM SEFD IFIN YIEA YMTM KIRN
Gene ID:3586
Uniprot:P22301
Cellular Location:Secreted
Calculated MW:20kDa
Observed MW:22kDa
Synonyms:CSIF, GVHDS, IL-10, IL10A, TGIF, IL10
Background:The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
UniProt Protein Function:IL10: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Belongs to the IL-10 family.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q31-q32

Cellular Component: extracellular space

Molecular Function:growth factor activity; cytokine activity; interleukin-10 receptor binding

Biological Process: B cell proliferation; negative regulation of chronic inflammatory response to antigenic stimulus; regulation of isotype switching; positive regulation of transcription, DNA-dependent; leukocyte chemotaxis; response to glucocorticoid stimulus; positive regulation of JAK-STAT cascade; negative regulation of B cell proliferation; negative regulation of membrane protein ectodomain proteolysis; response to insulin stimulus; negative regulation of interferon-alpha biosynthetic process; negative regulation of T cell proliferation; cytoplasmic sequestering of NF-kappaB; cell-cell signaling; positive regulation of MHC class II biosynthetic process; negative regulation of interferon-gamma production; response to molecule of bacterial origin; negative regulation of interleukin-6 production; hemopoiesis; negative regulation of tumor necrosis factor biosynthetic process; inflammatory response; aging; response to drug; response to inactivity; negative regulation of interleukin-12 production; positive regulation of B cell apoptosis; T-helper 2 type immune response; negative regulation of myeloid dendritic cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of cytokine secretion during immune response; regulation of gene expression; B cell differentiation; defense response to bacterium; negative regulation of MHC class II biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; receptor biosynthetic process; response to activity; regulation of sensory perception of pain; negative regulation of apoptosis; positive regulation of cytokine secretion

Disease: Graft-versus-host Disease, Susceptibility To; Rheumatoid Arthritis; Human Immunodeficiency Virus Type 1, Susceptibility To

NCBI Summary:The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]
UniProt Code:P22301
NCBI GenInfo Identifier:124292
NCBI Gene ID:3586
NCBI Accession:P22301.1
UniProt Related Accession:P22301
Molecular Weight:20,517 Da
NCBI Full Name:Interleukin-10
NCBI Synonym Full Names:interleukin 10
NCBI Official Symbol:IL10  
NCBI Official Synonym Symbols:CSIF; TGIF; GVHDS; IL-10; IL10A  
NCBI Protein Information:interleukin-10; T-cell growth inhibitory factor; cytokine synthesis inhibitory factor
UniProt Protein Name:Interleukin-10
UniProt Synonym Protein Names:Cytokine synthesis inhibitory factor; CSIF
Protein Family:Interleukin
UniProt Gene Name:IL10  
UniProt Entry Name:IL10_HUMAN
Anti-IL-10 Antibody (CAB2171)Western blot analysis of extracts of Jurkat cells, using IL10 antibody (CAB2171) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s.
Anti-IL-10 Antibody (CAB2171)Immunohistochemistry of paraffin-embedded mouse spleen using IL10 antibody (CAB2171) at dilution of 1:200 (40x lens).

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