The Integrin Beta 4 Polyclonal Antibody (CAB0857) is a valuable tool for researchers studying integrin beta 4, a cell surface protein involved in cell adhesion and migration. This antibody, produced in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. By binding to the integrin beta 4 protein, this antibody enables researchers to detect and analyze its expression in various cell types, making it an essential tool for research in cell biology and cancer metastasis.Integrin beta 4 is a critical component of hemidesmosomes, structures that anchor epithelial cells to the basement membrane.
Dysregulation of integrin beta 4 expression or function is associated with various diseases, including cancer and skin disorders. Studying integrin beta 4 with this antibody could provide insights into the mechanisms underlying these conditions and help identify potential therapeutic targets. Researchers interested in cell adhesion, migration, and cancer biology will find this antibody indispensable for their experiments.
Cell junction, Cell membrane, Lipid-anchor, Single-pass type I membrane protein, hemidesmosome.
Calculated MW:
202kDa
Observed MW:
202kDa
Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3691
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of PC-3, using Integrin beta 4 (ITGB4/CD104) Rabbit pAb (CAB0857) at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
