The Integrin Alpha 4 Polyclonal Antibody (CAB0696) is a valuable tool for researchers studying integrin alpha 4, a cell surface protein involved in cell adhesion and migration processes. This antibody, generated in rabbits, demonstrates high specificity and sensitivity in detecting integrin alpha 4 in human samples, making it suitable for experiments utilizing Western blotting techniques.Integrin alpha 4 plays a pivotal role in various physiological and pathological processes, including immune cell trafficking, tissue repair, and cancer metastasis. By targeting integrin alpha 4, researchers can gain insights into its molecular interactions and functions in different cell types, offering a better understanding of its potential implications in immunology, oncology, and tissue development studies.
With its ability to detect integrin alpha 4 expression, the Integrin Alpha 4 Polyclonal Antibody is an essential tool for investigating the mechanisms underlying integrin alpha 4-mediated cellular activities. Its application extends to a wide range of research areas, making it a valuable asset in exploring the role of integrin alpha 4 in various biological processes and disease conditions.
The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
3676
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of Jurkat, using Integrinalpha4(ITGA4/CD49d) antibody (CAB0696) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
