The Insulin Receptor Polyclonal Antibody (CAB16900) is a valuable tool for researchers studying the insulin receptor, a key player in metabolic regulation and cell signaling. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blotting applications. By binding to the insulin receptor protein, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for studies in diabetes, obesity, and other metabolic disorders.The insulin receptor plays a crucial role in regulating glucose and lipid metabolism, making it a target of interest in understanding diseases like type 2 diabetes and metabolic syndrome.
By studying the activity of the insulin receptor using this antibody, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets for treatment. With its high specificity and sensitivity, the Insulin Receptor Polyclonal Antibody is a valuable tool for advancing research in the field of metabolic health.
Product Name:
Insulin Receptor Rabbit Polyclonal Antibody
SKU:
CAB16900
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1281-1382 of human INSR (NP_000199.2).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
HHF5; CD220; Insulin Receptor
Positive Sample:
HepG2,HeLa,Mouse brain,Mouse kidney
Cellular Localization:
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
156kDa
Observed MW:
110kDa/220kDa
This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
3643
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Insulin Receptor Rabbit pAb (CAB16900) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
