The Insulin Receptor Polyclonal Antibody (CABP0044) is a vital tool for research involving the insulin receptor, a key player in the regulation of glucose metabolism and cell growth. This antibody, produced in rabbits, demonstrates high specificity for human samples and is validated for use in Western blot analyses. By binding to the insulin receptor protein, this antibody enables researchers to detect and analyze its expression in various cell types, making it an essential tool for studies in diabetes, obesity, and cancer research.The insulin receptor plays a crucial role in mediating the effects of insulin, a hormone that controls blood sugar levels and promotes cell growth.
Dysregulation of the insulin receptor pathway is implicated in various diseases, including type 2 diabetes and certain types of cancer. Understanding the function and regulation of the insulin receptor is essential for developing novel therapies and treatments for these conditions. The Insulin Receptor Polyclonal Antibody is a valuable resource for researchers aiming to unravel the molecular mechanisms underlying these diseases and identify potential targets for therapeutic intervention.
A synthetic phosphorylated peptide around Y1150 of human IGF1RINSR (NP_001073285.1).
Sequence:
TDYY R
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
IGF1R/INSR; Phospho-INSR-Y1150/IGF1R-Y1135
Positive Sample:
293,HepG2
Conjugate:
Unconjugated
Cellular Localization:
cytoplasm, nucleus, plasma membrane, T-tubule
Calculated MW:
154kDa/155kDa/156kDa
Observed MW:
80kDa
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]
Purification Method:
Affinity purification
Gene ID:
3480/3643
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of 293 and HepG2 cells, using Phospho-INSR-Y1150/IGF1R-Y1135 antibody (CABP0044) at 1:1000 dilution. 293 cells were treated by EGF (25μg/mL) for 30 minutes after serum-starvation overnight. HepG2 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
