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Anti-Insulin Receptor Antibody (CAB0005)

SKU:
CAB0005
Product Type:
Antibody
Reactivity:
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
  • Metabolism Antibodies 1 Anti-Insulin Receptor Antibody CAB0005
  • Metabolism Antibodies 1 Anti-Insulin Receptor Antibody CAB0005
€129 - €609
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Description

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Anti-Insulin Receptor Antibody (CAB0005)

The INSR Polyclonal Antibody (CAB0005) is a valuable tool for research involving the INSR protein, a key player in insulin signal transduction and regulation of glucose metabolism. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the INSR protein, this antibody enables precise detection and analysis in various cell types, making it an essential component for studies in diabetes research and metabolic disorders.

INSR, also known as the insulin receptor, is crucial for mediating the effects of insulin on glucose uptake and metabolism in tissues such as muscle, liver, and fat cells. Dysregulation of INSR signaling is associated with insulin resistance, a hallmark of type 2 diabetes and other metabolic conditions. Investigating the function and regulation of INSR is essential for understanding the underlying mechanisms of these diseases and developing targeted therapies to improve insulin sensitivity and metabolic health.

Antibody Name:Anti-Insulin Receptor Antibody
Antibody SKU:CAB0005
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1130-1230 of human Insulin Receptor (NP_000199.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Mouse, Rat
Positive Samples:Mouse liver, Rat liver
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1130-1230 of human Insulin Receptor (NP_000199.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PPTL QEMI QMAA EIAD GMAY LNAK KFVH RDLA ARNC MVAH DFTV KIGD FGMT RDIY ETDY YRKG GKGL LPVR WMAP ESLK DGVF TTSS DMWS FGVV LWEI T
Gene ID:3643
Uniprot:P06213
Cellular Location:Cell membrane, Single-pass type I membrane protein
Calculated MW:155kDa/156kDa
Observed MW:180kDa
Synonyms:CD220, HHF5, INSR
Background:This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosine residues, including the p85 regulatory subunit of PI3K and SHP2. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway, which is responsible for most of the metabolic actions of insulin, and the Ras-MAPK pathway, which regulates expression of some genes and cooperates with the PI3K pathway to control cell growth and differentiation. Binding of the SH2 domains of PI3K to phosphotyrosines on IRS1 leads to the activation of PI3K and the generation of phosphatidylinositol-(3, 4, 5)-triphosphate (PIP3), a lipid second messenger, which activates several PIP3-dependent serine/threonine kinases, such as PDPK1 and subsequently AKT/PKB. The net effect of this pathway is to produce a translocation of the glucose transporter SLC2A4/GLUT4 from cytoplasmic vesicles to the cell membrane to facilitate glucose transport. Moreover, upon insulin stimulation, activated AKT/PKB is responsible for: anti-apoptotic effect of insulin by inducing phosphorylation of BAD; regulates the expression of gluconeogenic and lipogenic enzymes by controlling the activity of the winged helix or forkhead (FOX) class of transcription factors. Another pathway regulated by PI3K-AKT/PKB activation is mTORC1 signaling pathway which regulates cell growth and metabolism and integrates signals from insulin. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 thereby activating mTORC1 pathway. The Ras/RAF/MAP2K/MAPK pathway is mainly involved in mediating cell growth, survival and cellular differentiation of insulin. Phosphorylated IRS1 recruits GRB2/SOS complex, which triggers the activation of the Ras/RAF/MAP2K/MAPK pathway. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). Isoform Short has a higher affinity for IGFII binding. When present in a hybrid receptor with IGF1R, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.
NCBI Summary:This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
UniProt Code:P06213
NCBI GenInfo Identifier:308153655
NCBI Gene ID:3643
NCBI Accession:P06213.4
UniProt Secondary Accession:P06213,Q17RW0, Q59H98, Q9UCB7, Q9UCB8, Q9UCB9,
UniProt Related Accession:P06213
Molecular Weight:155,146 Da
NCBI Full Name:Insulin receptor
NCBI Synonym Full Names:insulin receptor
NCBI Official Symbol:INSR  
NCBI Official Synonym Symbols:HHF5; CD220  
NCBI Protein Information:insulin receptor
UniProt Protein Name:Insulin receptor
UniProt Synonym Protein Names:CD_antigen: CD220
UniProt Gene Name:INSR  
Anti-Insulin Receptor Antibody (CAB0005)Western blot analysis of extracts of various cell lines, using Insulin Receptor antibody (CAB0005) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-Insulin Receptor Antibody (CAB0005)Immunohistochemistry of paraffin-embedded mouse liver using Insulin Receptor antibody (CAB0005) at dilution of 1:100 (40x lens).

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