The Insig1 Monoclonal Antibody is a powerful tool for research involving Insig1, a key regulator of cholesterol homeostasis and lipid metabolism. This antibody, generated using hybridoma technology, specifically targets Insig1 in human samples and is validated for use in various applications, including immunofluorescence and immunoprecipitation.Insig1 is a crucial protein involved in the regulation of cholesterol biosynthesis and degradation pathways, making it a potential target for therapeutic intervention in metabolic disorders such as atherosclerosis and obesity. By specifically binding to Insig1, this antibody allows for the detection and analysis of this important protein in cell culture and tissue samples, enabling researchers to investigate its role in lipid metabolism and related diseases.
With its high specificity and sensitivity, the Insig1 Monoclonal Antibody is an invaluable tool for studies in molecular biology, biochemistry, and lipid metabolism research. Its ability to target Insig1 with precision makes it a reliable choice for researchers looking to deepen their understanding of cholesterol regulation and develop novel therapies for metabolic diseases.
Product Name:
INSIG1 Monoclonal Antibody
SKU:
CAB23531
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant protein of human INSIG1.
Tested Applications:
IHC-PELISA
Recommended Dilution:
IHC-P,1:50 - 1:200
Synonyms:
CL6; INSIG1
Conjugate:
Unconjugated
Calculated MW:
30kDa
Observed MW:
Refer to figures
This gene encodes an endoplasmic reticulum membrane protein that regulates cholesterol metabolism, lipogenesis, and glucose homeostasis. The encoded protein has six transmembrane helices which contain an effector protein binding site. It binds the sterol-sensing domains of sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase), and is essential for the sterol-mediated trafficking of these two proteins. It promotes the endoplasmic reticulum retention of SCAP and the ubiquitin-mediated degradation of HMG-CoA reductase. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
3638
Clone Number:
ARC61103
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Immunohistochemistry analysis of paraffin-embedded rat liver using INSIG1 Rabbit mAb (CAB23531) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
