The INPP5E Polyclonal Antibody (CAB17735) is a valuable tool for research involving INPP5E, a phosphoinositide 5-phosphatase enzyme that plays a crucial role in regulating cellular signaling pathways. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the INPP5E protein, this antibody allows for the detection and analysis of INPP5E in various cell types, making it ideal for studies in cell biology and molecular signaling pathways.INPP5E is involved in the regulation of phosphoinositide metabolism, which is essential for various cellular processes such as membrane trafficking, cell growth, and cell survival.
Dysregulation of INPP5E has been linked to various diseases, including cancer, neurological disorders, and metabolic diseases. Studying the function and regulation of INPP5E is critical for understanding its role in disease pathogenesis and for developing targeted therapies.Overall, the INPP5E Polyclonal Antibody is a valuable tool for researchers investigating the function of INPP5E in cellular signaling pathways and its implications in disease biology. Its high specificity and reactivity make it a reliable choice for Western blot experiments to further our understanding of INPP5E biology and its potential as a therapeutic target.
Product Name:
INPP5E Rabbit Polyclonal Antibody
SKU:
CAB17735
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 510-630 of human INPP5E (NP_063945.2).
The protein encoded by this gene is an inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase. InsP3 5-phosphatases hydrolyze Ins(1,4,5)P3, which mobilizes intracellular calcium and acts as a second messenger mediating cell responses to various stimulation. Studies of the mouse counterpart suggest that this protein may hydrolyze phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,5-bisphosphate on the cytoplasmic Golgi membrane and thereby regulate Golgi-vesicular trafficking. Mutations in this gene cause Joubert syndrome; a clinically and genetically heterogenous group of disorders characterized by midbrain-hindbrain malformation and various associated ciliopathies that include retinal dystrophy, nephronophthisis, liver fibrosis and polydactyly. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
56623
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from SH-SY5Y cells, using INPP5E Rabbit pAb (CAB17735) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
