The ING3 Polyclonal Antibody (CAB5832) is a valuable tool for researchers studying the ING3 protein, which plays a critical role in gene regulation and DNA repair processes. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the ING3 protein, this antibody allows for the detection and analysis of ING3 expression in various cell types, making it ideal for studies in molecular biology and cancer research.
ING3, a member of the inhibitor of growth (ING) family, is known to interact with histone acetyltransferases and play a role in chromatin remodeling. Dysregulation of ING3 has been implicated in various diseases, including cancer, making it a promising target for therapeutic interventions. Understanding the function of ING3 and its role in gene regulation is crucial for developing targeted therapies aimed at treating cancer and other related conditions.
Product Name:
ING3 Rabbit Polyclonal Antibody
SKU:
CAB5832
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human ING3 (NP_938008.1).
The protein encoded by this gene is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Overexpression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Two alternatively spliced transcript variants encoding different isoforms have been observed.
Purification Method:
Affinity purification
Gene ID:
54556
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ING3 antibody (CAB5832) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
