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INCENP Rabbit Polyclonal Antibody (CAB0622)

SKU:
CAB0622
Product Type:
Antibody
Reactivity:
Human
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
€139 - €419

Description

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INCENP Rabbit Polyclonal Antibody (CAB0622)

The INCENP Polyclonal Antibody (CAB0622) is a key tool for researchers studying the INCENP protein, a crucial component of the chromosomal passenger complex involved in cell division. This polyclonal antibody, produced in rabbits, is highly specific for human samples and has been extensively validated for use in Western blot applications.INCENP is essential for proper chromosome segregation and cytokinesis during cell division, making it a key target for studies in cell biology and cancer research. The INCENP Polyclonal Antibody binds specifically to the INCENP protein, allowing for precise detection and analysis in a variety of cell types.

This antibody is an invaluable resource for researchers investigating the mechanisms of cell division and the role of INCENP in disease states such as cancer.With its high reactivity and specificity, the INCENP Polyclonal Antibody (CAB0622) is an essential tool for researchers seeking to further understand the functions and regulatory mechanisms of the INCENP protein in cellular processes.

Product Name:INCENP Rabbit Polyclonal Antibody
SKU:CAB0622
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 785-914 of human INCENP (NP_064623.2).
Sequence:ALNV TVDV QSPA CTSY QMTP QGHR APPK INPD NYGM DLNS DDST DDEA HPRK PIPT WARG TPLS QAII HQYY HPPN LLEL FGTI LPLD LEDI FKKS KPRY HKRT SSAV WNSP PLQG ARVP SSLA YSLK KH
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:INCENP
Positive Sample:HeLa,Jurkat,Rat thymus
Conjugate:Unconjugated
Cellular Localization:Chromosome, Cytoplasm, Nucleus, centromere, cytoskeleton, kinetochore, spindle.
Calculated MW:105kDa
Observed MW:120-140kDa

In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999 [PubMed 10369859]).

Purification Method:Affinity purification
Gene ID:3619
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using INCENP antibody (CAB0622) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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