The ILF3 Polyclonal Antibody (CAB2496) is a valuable tool for researchers studying the ILF3 protein, which plays a critical role in gene regulation and RNA processing. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to ILF3, this antibody allows for the detection and analysis of the protein in various cell types, making it ideal for studies in molecular biology, immunology, and cancer research.ILF3, also known as interleukin enhancer-binding factor 3, is involved in numerous cellular processes including transcription, RNA splicing, and protein translation.
Its diverse functions make it a key player in gene expression regulation and cell signaling pathways. Research into ILF3 is crucial for understanding its role in cellular physiology and disease development, particularly in cancer and autoimmune disorders.Utilizing the ILF3 Polyclonal Antibody (CAB2496) in your experiments can provide valuable insights into the role of ILF3 in various biological processes, furthering our understanding of gene regulation and potential therapeutic targets in disease.
Product Name:
ILF3 Rabbit Polyclonal Antibody
SKU:
CAB2496
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ILF3 (NP_036350.2).
This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
3609
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Hep G2 cells using ILF3 Rabbit pAb(CAB2496) at 1:1000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime:0.5s.
