The ILF2 Polyclonal Antibody (CAB5882) is a valuable tool for research involving the ILF2 protein, which plays a crucial role in gene regulation and mRNA processing. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the ILF2 protein, researchers can easily detect and analyze its expression in different cell types, making it ideal for studies in molecular biology and gene expression regulation.ILF2, also known as Interleukin Enhancer Binding Factor 2, is involved in a variety of cellular processes, including transcriptional regulation and RNA splicing.
Its importance in gene expression control makes it a key target for research aimed at understanding diseases such as cancer, neurodegenerative disorders, and developmental abnormalities. By investigating the function of ILF2, scientists can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic strategies.
Product Name:
ILF2 Rabbit Polyclonal Antibody
SKU:
CAB5882
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ILF2 (NP_004506.2).
The protein encoded by this gene is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The encoded 45 kDa protein (NF45, ILF2) forms a complex with the 90 kDa interleukin enhancer-binding factor 3 (NF90, ILF3), and this complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm, to repair DNA breaks by nonhomologous end joining, and to negatively regulate the microRNA processing pathway. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. Alternative splicing results in multiple transcript variants. Related pseudogenes have been found on chromosomes 3 and 14.
Purification Method:
Affinity purification
Gene ID:
3608
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ILF2 antibody (CAB5882) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (AbGn00020)._Exposure time: 300s.
