The ILF2 Polyclonal Antibody (CAB13320) is a valuable tool for researchers studying ILF2, a key protein involved in RNA binding and transcription regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the ILF2 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential component of studies in molecular biology and gene expression.
ILF2, also known as interleukin enhancer-binding factor 2, plays a crucial role in regulating gene expression, particularly in immune responses and cellular growth. Its involvement in transcriptional control makes ILF2 a significant target for research into various diseases, including cancer, viral infections, and autoimmune disorders. By understanding the function of ILF2, researchers can gain insights into disease mechanisms and potentially identify new therapeutic targets for treating these conditions.
Product Name:
ILF2 Rabbit Polyclonal Antibody
SKU:
CAB13320
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ILF2 (NP_004506.2).
The protein encoded by this gene is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The encoded 45 kDa protein (NF45, ILF2) forms a complex with the 90 kDa interleukin enhancer-binding factor 3 (NF90, ILF3), and this complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm, to repair DNA breaks by nonhomologous end joining, and to negatively regulate the microRNA processing pathway. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. Alternative splicing results in multiple transcript variants. Related pseudogenes have been found on chromosomes 3 and 14.
Purification Method:
Affinity purification
Gene ID:
3608
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ILF2 antibody (CAB13320) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5min.
