The Human CD25 Monoclonal Antibody (FITC Conjugated, BC96) is a valuable tool for research involving CD25, a cell surface marker associated with regulatory T cells and immune regulation. This antibody, conjugated with FITC for fluorescence detection, is specifically designed for use in flow cytometry applications, enabling the identification and analysis of CD25-expressing cells in human samples.CD25, also known as the interleukin-2 receptor alpha chain, is a crucial component in the activation and function of regulatory T cells, which play a key role in maintaining immune tolerance and preventing autoimmune responses. By targeting CD25, researchers can better understand the mechanisms of immune regulation and investigate the role of regulatory T cells in various physiological and pathological conditions.
With its high specificity and sensitivity, the Human CD25 Monoclonal Antibody (FITC Conjugated, BC96) offers reliable and accurate detection of CD25 expression, making it an essential tool for studies in immunology, autoimmunity, transplantation, and cancer research. By utilizing this antibody, researchers can gain insights into the role of CD25-expressing cells in immune responses and potentially identify new therapeutic targets for manipulating immune function.
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
P01589
Gene ID:
3559
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.