The IL1B Polyclonal Antibody (CAB16288) is a valuable tool for researchers studying the interleukin-1 beta protein, a key player in the immune response and inflammation pathways. This antibody, generated in rabbits, is highly specific to human IL1B and has been validated for use in Western blot applications, enabling accurate detection and analysis of IL1B protein levels in various cell types.IL1B, a pro-inflammatory cytokine, is involved in a wide range of physiological processes, including immune response, inflammation, and tissue repair.
Dysregulation of IL1B signaling has been implicated in various diseases, such as autoimmune disorders, infectious diseases, and inflammatory conditions. By targeting IL1B with this antibody, researchers can gain insights into the role of IL1B in health and disease, paving the way for the development of novel therapeutics targeting IL1B signaling pathways.
Product Name:
IL1β Rabbit Polyclonal Antibody
SKU:
CAB16288
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 70-170 of human IL1β (NP_000567.1).
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
Purification Method:
Affinity purification
Gene ID:
3553
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from THP-1 cells, using IL1β Rabbit pAb (CAB16288) at 1:1000 dilution.THP-1 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
