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Anti-Il1b Antibody (CAB20527)

SKU:
CAB20527
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Size:
20uL
€139
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Description

system_update_alt Datasheet system_update_alt MSDS
Product Name:Il1b Rabbit Polyclonal Antibody
SKU:CAB20527
Size:20uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human, Mouse, Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 118-269 of mouse IL1β (NP_032387.1).
Sequence:VPIR QLHY RLRD EQQK SLVL SDPY ELKA LHLN GQNI NQQV IFSMS FVQG EPSN DKIP VALG LKGK NLYL SCVM KDGT PTLQ LESV DPKQ YPKK KMEK RFVF NKIE VKSK VEFE SAEF PNWY ISTS QAEE HKPV FLGN NSGQ DIID FTMES VSS
Tested Applications: WB IHC-P IF/ICC ELISA
Recommended Dilution:
  • WB, 1:500 - 1:1000
  • IHC-P, 1:50 - 1:200
  • IF/ICC, 1:50 - 1:200
Synonyms:IL-1 beta, IL1β, Interleukin-1 beta
Positive Sample:THP-1, Raw 264.7, Recombinant Mouse IL-1 beta Protein
Conjugate:Unconjugated
Cellular Localization:cytosol, extracellular region, extracellular space, lysosome
Calculated MW:31kDa
Observed MW:31kDa//17kDa

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. The encoded protein plays a role in thymocyte proliferation and is involved in the inflammatory response.

Purification Method:Affinity purification
Gene ID:16176
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Product Image Western blot analysis of various lysates using IL1β Rabbit pAb (CAB20527) at 1:1000 dilution. THP-1 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (AbGn00021).
Exposure time: 180s.

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