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Anti-Il1b Antibody (CAB20527)

SKU:
CAB20527
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Size:
20uL
€139
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Description

system_update_alt Datasheet system_update_alt MSDS

Anti-Il1b Antibody (CAB20527)

The Anti-IL1B Antibody (CAB20527) is a vital tool for researchers studying IL1B, a key pro-inflammatory cytokine involved in various inflammatory processes. This polyclonal antibody is specifically designed to target IL1B, a critical mediator of the immune response, and is highly reactive with human samples. Validated for use in Western blot applications, this antibody enables the detection and analysis of IL1B protein in a variety of cell types.IL1B, also known as interleukin-1 beta, plays a crucial role in the regulation of immune and inflammatory responses. Dysregulation of IL1B has been implicated in numerous diseases, including autoimmune disorders, inflammatory conditions, and even cancer.

Understanding the function of IL1B is essential for developing targeted therapies that can modulate the immune response in these disease contexts.With its high specificity and reactivity, the Anti-IL1B Antibody is a valuable tool for researchers seeking to explore the role of IL1B in immune regulation and disease pathogenesis. Whether studying inflammatory pathways or developing novel therapeutic strategies, this antibody is a crucial component for advancing research in immunology and beyond.

Product Name:Il1b Rabbit Polyclonal Antibody
SKU:CAB20527
Size:20uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human, Mouse, Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 118-269 of mouse IL1β (NP_032387.1).
Sequence:VPIR QLHY RLRD EQQK SLVL SDPY ELKA LHLN GQNI NQQV IFSMS FVQG EPSN DKIP VALG LKGK NLYL SCVM KDGT PTLQ LESV DPKQ YPKK KMEK RFVF NKIE VKSK VEFE SAEF PNWY ISTS QAEE HKPV FLGN NSGQ DIID FTMES VSS
Tested Applications: WB IHC-P IF/ICC ELISA
Recommended Dilution:
  • WB, 1:500 - 1:1000
  • IHC-P, 1:50 - 1:200
  • IF/ICC, 1:50 - 1:200
Synonyms:IL-1 beta, IL1β, Interleukin-1 beta
Positive Sample:THP-1, Raw 264.7, Recombinant Mouse IL-1 beta Protein
Conjugate:Unconjugated
Cellular Localization:cytosol, extracellular region, extracellular space, lysosome
Calculated MW:31kDa
Observed MW:31kDa//17kDa

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. The encoded protein plays a role in thymocyte proliferation and is involved in the inflammatory response.

Purification Method:Affinity purification
Gene ID:16176
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Product Image Western blot analysis of various lysates using IL1β Rabbit pAb (CAB20527) at 1:1000 dilution. THP-1 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (AbGn00021).
Exposure time: 180s.

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