The IL18 Polyclonal Antibody (CAB20473) is a valuable tool for researchers investigating interleukin-18 (IL-18), a proinflammatory cytokine involved in various immune responses and inflammatory processes. This antibody is produced in rabbits and exhibits high reactivity with human samples, making it a reliable choice for Western blotting applications. By binding to IL-18, this antibody enables accurate detection and analysis of the protein in different cell types, opening up opportunities for studies in immunology, inflammation, and related fields.
IL-18 is known for its role in promoting inflammation and immune responses, making it a key player in diseases such as autoimmune disorders, infectious diseases, and inflammatory conditions. By targeting IL-18 with this specific antibody, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets. Understanding the functions of IL-18 is essential for developing strategies to modulate immune responses and combat various inflammatory disorders.
Product Name:
IL18 Rabbit Polyclonal Antibody
SKU:
CAB20473
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 36-192 of mouse IL18 (NP_032386.1).
RAW264.7,Mouse spleen,Rat brain,Recombinant Human IL-18 Protein
Conjugate:
Unconjugated
Cellular Localization:
apical plasma membrane, cytoplasm, extracellular space
Calculated MW:
22kDa
Observed MW:
22kDa/18kDa
Enables cytokine activity. Involved in lipid homeostasis; positive regulation of cell differentiation; and positive regulation of cold-induced thermogenesis. Acts upstream of or within several processes, including interleukin-18-mediated signaling pathway; positive regulation of NIK/NF-kappaB signaling; and positive regulation of interferon-gamma production. Located in extracellular space. Is expressed in several structures, including back skin; dorsal aorta; gut; lymphatic system; and reproductive system. Used to study atopic dermatitis. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Orthologous to human IL18 (interleukin 18).
Purification Method:
Affinity purification
Gene ID:
16173
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using IL18 Rabbit pAb (CAB20473) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
