Anti-IL-18 Antibody (CAB16737)

Antibody Name:	IL18 Rabbit Polyclonal Antibody
Antibody SKU:	CAB16737
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant proteinof human IL18.

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	mouse liver, rat lung, rat spleen, rat kidney

Immunogen:	Recombinant proteinof human IL18.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	3606
Uniprot:	Q14116
Cellular Location:	Secreted
Calculated MW:	21kDa/22kDa
Observed MW:	23kDa

Synonyms:	IL18, IGIF, IL-18, IL-1g, IL1F4
Background:	The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	IL18: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Belongs to the IL-1 family.
UniProt Protein Details:	Protein type:Cytokine Chromosomal Location of Human Ortholog: 11q22.2-q22.3 Cellular Component: extracellular space; extracellular region; cytosol Molecular Function:protein binding; cytokine activity Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of NK T cell proliferation; natural killer cell activation; positive regulation of NF-kappaB import into nucleus; positive regulation of activated T cell proliferation; chemokine biosynthetic process; cell-cell signaling; lipopolysaccharide-mediated signaling pathway; granulocyte macrophage colony-stimulating factor biosynthetic process; angiogenesis; inflammatory response; positive regulation of interleukin-17 production; regulation of cell adhesion; positive regulation of natural killer cell proliferation; T-helper 1 type immune response; T-helper 2 type immune response; interleukin-2 biosynthetic process; MAPKKK cascade; interleukin-13 biosynthetic process; sleep; positive regulation of tissue remodeling; positive regulation of interferon-gamma production; interferon-gamma biosynthetic process; immune response; negative regulation of myoblast differentiation; positive regulation of inflammatory response
NCBI Summary:	The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]
UniProt Code:	Q14116
NCBI GenInfo Identifier:	3219817
NCBI Gene ID:	3606
NCBI Accession:	Q14116.1
UniProt Secondary Accession:	Q14116,O75599, Q6FGY3, Q6WWJ7,
UniProt Related Accession:	Q14116
Molecular Weight:	21,896 Da
NCBI Full Name:	Interleukin-18
NCBI Synonym Full Names:	interleukin 18
NCBI Official Symbol:	IL18
NCBI Official Synonym Symbols:	IGIF; IL-18; IL-1g; IL1F4
NCBI Protein Information:	interleukin-18; IL-1 gamma; iboctadekin; interleukin-1 gamma; IFN-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)
UniProt Protein Name:	Interleukin-18
UniProt Synonym Protein Names:	Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma
Protein Family:	Interleukin
UniProt Gene Name:	IL18
UniProt Entry Name:	IL18_HUMAN

	Western blot analysis of extracts of various cell lines, using IL18 antibody (CAB16737) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of C6 cells using IL18 antibody (CAB16737) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using IL18 antibody (CAB16737) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using IL18 antibody (CAB16737) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-IL-18 Antibody (CAB1115)[KO Validated]	Human IL-18 ELISA Kit
	Human IL-18 CLIA Kit (HUES00188)
	Human IL-18 ELISA Kit (HUES01492)
	Human IL-18 ELISA Kit (HUEB0024)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)