The IL12B Polyclonal Antibody (CAB13024) is a valuable tool for researchers studying IL12B, a cytokine involved in immune responses and inflammation. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the IL12B protein, this antibody enables accurate detection and analysis of IL12B in various cell types, making it ideal for studies in immunology and inflammatory diseases.IL12B, also known as interleukin-12 subunit beta, plays a critical role in the regulation of immune responses and the development of Th1 cells.
Dysregulation of IL12B has been implicated in various autoimmune diseases and inflammatory conditions, making it an important target for research aimed at understanding and treating these conditions. By studying the function and expression of IL12B, researchers can gain valuable insights into the mechanisms underlying immune responses and inflammation, paving the way for the development of novel therapeutic strategies.
Product Name:
IL12B Rabbit Polyclonal Antibody
SKU:
CAB13024
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 23-328 of human IL12B (NP_002178.2).
This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.
Purification Method:
Affinity purification
Gene ID:
3593
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using IL12B Rabbit pAb (CAB13024) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
