The IL-2 Polyclonal Antibody (CAB16898) is a valuable tool for research involving Interleukin-2 (IL-2), a cytokine that plays a crucial role in the regulation of immune responses. This antibody, raised in rabbits, is highly specific to IL-2 and is validated for use in various applications such as Western blot and Immunohistochemistry.IL-2 is involved in the proliferation and differentiation of T cells, making it a key molecule in the immune system's defense against pathogens. Dysregulation of IL-2 signaling has been implicated in various diseases, including autoimmune disorders and cancer.
By utilizing this antibody, researchers can study the expression and activity of IL-2 in different cell types and tissues, providing valuable insights into the immune response and potential therapeutic targets.Overall, the IL-2 Polyclonal Antibody (CAB16898) is an essential tool for studying the role of IL-2 in immune regulation, making it ideal for research in immunology, oncology, and autoimmune diseases.
Product Name:
IL2 Rabbit Polyclonal Antibody
SKU:
CAB16898
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 21-153 of IL2 (NP_000577.2).
Jurkat treated by TPA ,A23187 and Brefeldin A,Recombinant Human IL2 Protein
Cellular Localization:
Secreted.
Calculated MW:
18kDa
Observed MW:
14-17kDa
This gene is a member of the interleukin 2 (IL2) cytokine subfamily which includes IL4, IL7, IL9, IL15, IL21, erythropoietin, and thrombopoietin. The protein encoded by this gene is a secreted cytokine produced by activated CD4+ and CD8+ T lymphocytes, that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2R) is a heterotrimeric protein complex whose gamma chain is also shared by IL4 and IL7. The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
Purification Method:
Affinity purification
Gene ID:
3558
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using IL2 Rabbit pAb (CAB16898) at 1:1000 dilution.Jurkat cells were treated by TPA (40 nM),A23187 (2μM) and Brefeldin A (300 ng / ml) for 0-24hoursSecondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
