Western blot analysis of various lysates, using IL-1RAcP antibody (CAB22286) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The IL-1RAcP Monoclonal Antibody (CAB22286) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, produced in mice, is highly specific and sensitive for detecting IL1RACP in human samples. Validated for use in various applications, including Western blot and immunohistochemistry, this antibody allows for precise and accurate detection of IL1RACP in different cell types.IL1RACP, also known as IL-1R accessory protein, plays a crucial role in modulating the activity of the IL-1 signaling pathway, which is involved in inflammation and immune responses. Dysregulation of IL-1 signaling has been implicated in various diseases, including autoimmune disorders, inflammatory conditions, and cancer.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
IL-1RAcP Monoclonal Antibody
SKU:
CAB22286
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC56983
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IL1R3, C3orf13, IL-1RAcP
Positive Sample:
KARPAS-299, Mouse lung, Rat brain
Cellular Localization:
Cell Membrane, Secreted.
Calculated MW:
65kDa
Observed MW:
78kDa
This gene encodes a component of the interleukin 1 receptor complex, which initiates signalling events that result in the activation of interleukin 1-responsive genes. Alternative splicing of this gene results in membrane-bound and soluble isoforms differing in their C-terminus. The ratio of soluble to membrane-bound forms increases during acute-phase induction or stress.
Purification Method
Affinity purification
Gene ID
3556
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using IL-1RAcP Rabbit mAb (CAB22286) at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from Rat brain, using IL-1RAcP Rabbit mAb (CAB22286) at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
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