The IKZF1 Polyclonal Antibody (CAB1850) is a powerful tool for researchers studying IKZF1, a key transcription factor involved in immune cell development and function. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, allowing for reliable detection in Western blot assays.IKZF1, also known as Ikaros family zinc finger 1, plays a crucial role in the regulation of immune responses, particularly in the development and differentiation of lymphoid cells. Dysregulation of IKZF1 has been implicated in various immune-related disorders, including leukemia and autoimmune diseases.
By targeting IKZF1, researchers can gain insights into the mechanisms underlying these conditions and potentially identify novel therapeutic targets.With its ability to accurately detect IKZF1 protein expression in a variety of cell types, the CAB1850 antibody is an invaluable tool for studies in immunology, cancer biology, and beyond. By unraveling the intricate functions of IKZF1, researchers can pave the way for innovative treatments and interventions in the field of immune-mediated diseases.
Product Name:
Ikaros Rabbit Polyclonal Antibody
SKU:
CAB1850
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-270 of human Ikaros (NP_001207694.1).
This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL).
Purification Method:
Affinity purification
Gene ID:
10320
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Raji cells, using Ikaros Rabbit pAb (CAB1850) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
