Ikaros Rabbit Polyclonal Antibody (CAB1850)
- SKU:
- CAB1850
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Description
Product Name: | Ikaros Rabbit Polyclonal Antibody |
SKU: | CAB1850 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-270 of human Ikaros (NP_001207694.1). |
Sequence: | MDAD EGQD MSQV SGKE SPPV SDTP DEGD EPMP IPED LSTT SGGQ QSSK SDRV VASN VKVE TQSD EENG RACE MNGE ECAE DLRM LDAS GEKM NGSH RDQG SSAL SGVG GIRL PNGK LKCD ICGI ICIG PNVL MVHK RSHT GERP FQCN QCGA SFTQ KGNL LRHI KLHS GEKP FKCH LCNY ACRR RDAL TGHL RTHS VIKE ETNH SEMA EDLC KIGS ERSL VLDR LASN VAKR KSSM PQKF LGDK GLSD TPYD SSAS YEKE NEMM KSHV MD |
Tested Applications: | WB IF/ICC ChIP ELISA |
Recommended Dilution: | WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 ChIP,5μg antibody for 10μg-15μg of Chromatin |
Synonyms: | IK1; LYF1; LyF-1; CVID13; IKAROS; PPP1R92; PRO0758; ZNFN1A1; Hs.54452; Ikaros |
Positive Sample: | Raji |
Conjugate: | Unconjugated |
Cellular Localization: | Cytoplasm, Nucleus, Nucleus. |
Calculated MW: | 58kDa |
Observed MW: | 50-70kDa |
This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL).
Purification Method: | Affinity purification |
Gene ID: | 10320 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |