The IKBKE Polyclonal Antibody (CAB16470) is specifically designed for research involving IKBKE, a protein kinase involved in various cellular processes, including immune response and inflammation regulation. This antibody, raised in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. It targets the IKBKE protein, facilitating detection and analysis in different cell types, making it an ideal tool for studies in immunology and cancer research. IKBKE, also known as inhibitor of nuclear factor kappa-B kinase subunit epsilon, is a crucial player in the activation of NF-κB, a key transcription factor involved in the expression of genes related to inflammation and immune response.
Dysregulation of IKBKE has been implicated in various diseases, including cancer, inflammatory disorders, and autoimmune conditions. Understanding the role of IKBKE in these diseases is essential for developing targeted therapies that can modulate its activity and potentially improve patient outcomes.
Product Name:
IKKε Rabbit Polyclonal Antibody
SKU:
CAB16470
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 460-620 of human IKKε (NP_001180251.1).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
IKKE; IKKI; IKK-E; IKK-i; IKKε
Positive Sample:
K-562
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus, PML body.
Calculated MW:
80kDa
Observed MW:
110kDa
IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).
Purification Method:
Affinity purification
Gene ID:
9641
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Immunoprecipitation analysis of 200 μg extracts of K-562 cells, using 3 μg IKKε antibody (CAB16470). Western blot was performed from the immunoprecipitate using IKKε antibody (CAB16470) at a dilution of 1:1000.
