The IKKbeta Antibody (CAB2087) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through immunization of rabbits, exhibits high reactivity with human samples and is suitable for Western blot applications. By specifically binding to the IKBKB protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an essential component for studies in immunology and cancer research.IKBKB, also known as inhibitor of nuclear factor kappa-B kinase subunit beta, plays a crucial role in the activation of the NF-kappa-B signaling pathway, which is involved in various cellular processes such as inflammation, immune responses, and cell survival.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
IKKbeta Antibody
SKU:
CAB2087
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene.
Purification Method
Affinity purification
Gene ID
3551
RRID
AB_2764107
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with IKKβ siRNA using IKKβ Rabbit pAb (CAB2087) at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 20 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020) .Exposure time: 10 s.
Immunofluorescence analysis of NIH/3T3 cells using IKKβ Rabbit pAb (CAB2087) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
