Anti-IkBAlpha Antibody (CAB11474)
- SKU:
- CAB11474
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Frequently bought together:
Description
Anti-IkBAlpha Antibody (CAB11474)
The IKBalpha Polyclonal Antibody (CAB11474) is a valuable tool for researchers studying the protein IKBalpha, which plays a crucial role in the regulation of the NF-kappaB signaling pathway. This rabbit polyclonal antibody is highly specific and reactive with human samples, making it ideal for use in Western blot applications.IKBalpha is a key inhibitor of NF-kappaB, a transcription factor that regulates the expression of genes involved in immune responses, inflammation, cell survival, and proliferation. Dysregulation of the NF-kappaB pathway is implicated in various diseases, including cancer and inflammatory disorders.
By targeting IKBalpha, researchers can gain insights into the mechanisms underlying these diseases and potentially develop new therapeutic approaches.The IKBalpha Polyclonal Antibody enables the detection and analysis of IKBalpha protein levels in different cell types, providing researchers with a valuable tool for investigating the role of IKBalpha in various biological processes. Its high specificity and sensitivity make it a reliable choice for studies in immunology, oncology, and inflammation research.
| Antibody Name: | Anti-IkBAlpha Antibody |
| Antibody SKU: | CAB11474 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human IkBAlpha (NP_065390.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | DU145, HeLa, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human IkBAlpha (NP_065390.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MFQA AERP QEWA MEGP RDGL KKER LLDD RHDS GLDS MKDE EYEQ MVKE LQEI RLEP QEVP RGSE PWKQ QLTE DGDS FLHL AIIH EEKA LTME VIRQ VKGD LAFL NFQN NLQQ TPLH LAVI TNQP EIAE ALLG AGCD PELR DFRG NTPL HLAC EQGC LASV GVLT QSCT TPHL HSIL KATN YNGH TCLH LASI HGYL GIVE LLVS LGAD VNAQ EPCN GRTA LHLA VDLQ NPDL VSLL LKCG ADVN RVTY QGYS PYQL TWGR PSTR IQQQ LGQL TLEN LQML PESE DEES YDTE SEFT EFTE DELP YDDC VFGG QRLT L |
| Gene ID: | 4792 |
| Uniprot: | P25963 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 35kDa |
| Observed MW: | 36kDa |
| Synonyms: | IKBA, MAD-3, NFKBI, IKB alpha, NFKBIA, EDAID2 |
| Background: | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. |
| UniProt Protein Function: | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. |
| NCBI Summary: | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011] |
| UniProt Code: | P25963 |
| NCBI GenInfo Identifier: | 126682 |
| NCBI Gene ID: | 4792 |
| NCBI Accession: | P25963.1 |
| UniProt Secondary Accession: | P25963,B2R8L6, |
| UniProt Related Accession: | P25963 |
| Molecular Weight: | 35,609 Da |
| NCBI Full Name: | NF-kappa-B inhibitor alpha |
| NCBI Synonym Full Names: | NFKB inhibitor alpha |
| NCBI Official Symbol: | NFKBIAÂ Â |
| NCBI Official Synonym Symbols: | IKBA; MAD-3; NFKBIÂ Â |
| NCBI Protein Information: | NF-kappa-B inhibitor alpha |
| UniProt Protein Name: | NF-kappa-B inhibitor alpha |
| UniProt Synonym Protein Names: | I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3 |
| Protein Family: | NF-kappa-B inhibitor |
| UniProt Gene Name: | NFKBIAÂ Â |
| UniProt Entry Name: | IKBA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IkBalpha antibody (CAB11474) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 25s. |
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