IGHA2 Antibody (PACO59752)
- SKU:
- PACO59752
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
IGHA2 Antibody (PACO59752)
The IghA2 Antibody (PACO59752) is a valuable tool for researchers studying the immunoglobulin A (IgA) antibody subclass. This polyclonal antibody is raised in rabbits and is highly reactive with human samples, making it ideal for use in a variety of research applications.IgA antibodies play a crucial role in mucosal immunity, providing defense against pathogens at mucosal surfaces such as the respiratory and gastrointestinal tracts. The IghA2 antibody specifically targets the IgA2 subclass, which is important for protecting against bacterial infections and maintaining a healthy immune system.This antibody can be used in Western blot applications to detect and analyze IgA2 proteins in various cell types, allowing researchers to investigate the role of IgA2 in immune responses.
By understanding the function of IgA2 antibodies, researchers can gain insights into immune system function and develop therapies for diseases related to mucosal immunity.Overall, the IghA2 Antibody (PACO59752) is a valuable tool for researchers studying mucosal immunity and IgA antibodies, offering insights into immune responses and potential therapeutic targets for infectious diseases and autoimmune disorders.
| Antibody Name: | IGHA2 Antibody (PACO59752) |
| Antibody SKU: | PACO59752 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Immunoglobulin heavy constant α 2 protein (36-111AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO59752 diluted at 1:300 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of HepG2 cells with PACO59752 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen. Ig α is the major immunoglobulin class in body secretions. |
| Synonyms: | Immunoglobulin heavy constant α 2 (Ig alpha-2 chain C region) (Ig alpha-2 chain C region BUT) (Ig alpha-2 chain C region LAN), IGHA2 |
| UniProt Protein Function: | IGHA2: Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 14q32.33 Cellular Component: extracellular space; extracellular region Molecular Function:antigen binding Biological Process: retinal homeostasis; receptor-mediated endocytosis; glomerular filtration; antibacterial humoral response; immune response |
| UniProt Code: | P01877 |
| NCBI GenInfo Identifier: | 218512088 |
| NCBI Gene ID: | 3494 |
| NCBI Accession: | P01877.3 |
| UniProt Related Accession: | P01877 |
| Molecular Weight: | 36,526 Da |
| NCBI Full Name: | Ig alpha-2 chain C region, partial |
| NCBI Synonym Full Names: | immunoglobulin heavy constant alpha 2 (A2m marker) |
| NCBI Official Symbol: | IGHA2Â Â |
| UniProt Protein Name: | Ig alpha-2 chain C region |
| UniProt Gene Name: | IGHA2Â Â |
| UniProt Entry Name: | IGHA2_HUMAN |
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