The IGF2BP3 Polyclonal Antibody (CAB6099) is a valuable tool for researchers studying IGF2BP3, a protein involved in cancer progression and stem cell maintenance. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.IGF2BP3, also known as IMP3, is a member of the insulin-like growth factor-2 mRNA-binding protein family and plays a critical role in post-transcriptional regulation of gene expression. It is overexpressed in various cancers, including pancreatic, lung, and ovarian cancer, and its expression has been linked to poor prognosis in cancer patients.
By targeting IGF2BP3, researchers can investigate its role in tumor growth, metastasis, and drug resistance, providing valuable insights into potential therapeutic strategies for combating cancer. The IGF2BP3 Polyclonal Antibody is a crucial tool for these studies, enabling the detection and analysis of IGF2BP3 protein levels in cancer cell lines and tissues.
Product Name:
IGF2BP3 Rabbit Polyclonal Antibody
SKU:
CAB6099
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 300-579 of human IGF2BP3 (NP_006538.2).
WB,1:100 - 1:500 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
KOC; CT98; IMP3; KOC1; IMP-3; VICKZ3; IGF2BP3
Positive Sample:
HeLa,T-47D,Rat testis
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
64kDa
Observed MW:
64kDa
The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes.
Purification Method:
Affinity purification
Gene ID:
10643
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using IGF2BP3 Rabbit pAb (CAB6099) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
