The IGF2BP3 Polyclonal Antibody (CAB4444) is a powerful tool for researchers studying IGF2BP3, a protein involved in mRNA localization and stabilization. This antibody, generated in rabbits, is highly specific for human samples and is validated for use in Western blotting applications. It binds specifically to IGF2BP3, allowing for accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in molecular biology and cancer research.IGF2BP3, also known as insulin-like growth factor 2 mRNA-binding protein 3, plays a critical role in post-transcriptional regulation of gene expression and has been implicated in the progression of various cancers.
Understanding the function and regulation of IGF2BP3 is essential for advancing our knowledge of cancer biology and identifying potential therapeutic targets. The IGF2BP3 Polyclonal Antibody is a valuable tool for researchers interested in unraveling the complexities of this important protein and its role in disease development.
Product Name:
IGF2BP3 Rabbit Polyclonal Antibody
SKU:
CAB4444
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300-579 of human IGF2BP3 (NP_006538.2).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
KOC; CT98; IMP3; KOC1; IMP-3; VICKZ3; IGF2BP3
Positive Sample:
HepG2,A-549,293T,HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
64kDa
Observed MW:
70kDa
The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes.
Purification Method:
Affinity purification
Gene ID:
10643
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using IGF2BP3 Rabbit pAb (CAB4444) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
