The IGF1R Polyclonal Antibody (CAB0243) is designed for research involving the insulin-like growth factor 1 receptor (IGF1R), a cell surface receptor involved in cell growth, differentiation, and survival. The antibody, raised in rabbits, is highly reactive with human samples and is validated for use in various applications including Western blot, immunofluorescence, and immunohistochemistry.IGF1R plays a key role in regulating cell proliferation and survival, making it a critical target for cancer research and therapeutics.
Abnormal activation of IGF1R has been implicated in various cancers, and targeting this receptor has shown promise in inhibiting tumor growth.This antibody enables the detection and analysis of IGF1R expression in different cell types and tissues, providing valuable insights into its role in cancer development and progression. Its high specificity and sensitivity make it an essential tool for studying the molecular mechanisms underlying cancer biology and for developing targeted therapies aimed at blocking IGF1R signaling.
Product Name:
IGF1R Rabbit Polyclonal Antibody
SKU:
CAB0243
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1201-1367 of human IGF1R (NP_000866.1).
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
155kDa
Observed MW:
100kDa/
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3480
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and IGF1R knockdown (KD) 293T cells using [KD Validated] IGF1R Rabbit pAb (CAB0243) at 1:3000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
