The IFT74 Polyclonal Antibody (CAB12672) is a valuable tool for researchers studying the IFT74 protein, which plays a key role in ciliary function and intracellular transport processes. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the IFT74 protein, this antibody enables the detection and analysis of IFT74 in a variety of cell types, making it essential for research in cell biology, ciliopathy, and developmental biology.
The IFT74 protein is a crucial component of the intraflagellar transport (IFT) complex, responsible for the movement of proteins along the ciliary axoneme. Defects in IFT proteins, including IFT74, have been linked to ciliopathies, a group of disorders characterized by abnormal cilia structure and function. Understanding the role of IFT74 in ciliary transport is essential for unraveling the molecular mechanisms underlying ciliopathies and developing targeted therapeutics for these conditions.
Product Name:
IFT74 Rabbit Polyclonal Antibody
SKU:
CAB12672
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-372 of human IFT74 (NP_001092694.1).
This gene encodes a core intraflagellar transport (IFT) protein which belongs to a multi-protein complex involved in the transport of ciliary proteins along axonemal microtubules. IFT proteins are found at the base of the cilium as well as inside the cilium, where they assemble into long arrays between the ciliary base and tip. This protein, together with intraflagellar transport protein 81, binds and transports tubulin within cilia and is required for ciliogenesis. Naturally occurring mutations in this gene are associated with amyotrophic lateral sclerosis--frontotemporal dementia and Bardet-Biedl Syndrome.
Purification Method:
Affinity purification
Gene ID:
80173
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using IFT74 Rabbit pAb (CAB12672) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
