The IFIH1 Polyclonal Antibody (CAB13645) is a valuable tool for researchers studying the IFIH1 gene, which encodes an important protein involved in the innate immune response to viral infections. This antibody, produced in rabbits, is highly specific to human IFIH1 and has been validated for use in Western blot applications. By targeting the IFIH1 protein, this antibody enables researchers to detect and analyze IFIH1 expression in various cell types, providing valuable insights into the role of IFIH1 in immune signaling pathways.IFIH1, also known as MDA5 (melanoma differentiation-associated protein 5), is a key player in antiviral immunity, sensing viral RNA and triggering immune responses to combat viral infections.
Dysregulation of IFIH1 has been implicated in autoimmune diseases and chronic inflammatory conditions, making it a promising target for therapeutic interventions. By studying IFIH1 function using the IFIH1 Polyclonal Antibody, researchers can uncover novel insights into the mechanisms of viral immunity and potential therapeutic strategies for treating viral infections and immune-related disorders.
Product Name:
IFIH1 Rabbit Polyclonal Antibody
SKU:
CAB13645
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human IFIH1 (NP_071451.2).
IFIH1 encodes MDA5 which is an intracellular sensor of viral RNA that triggers the innate immune response. Sensing RNA length and secondary structure, MDA5 binds dsRNA oligonucleotides with a modified DExD/H-box helicase core and a C-terminal domain, thus leading to a proinflammatory response that includes interferons. It has been shown that Coronaviruses (CoVs) as well as various other virus families, are capable of evading the MDA5-dependent interferon response, thus impeding the activation of the innate immune response to infection. MDA5 has also been shown to play an important role in enhancing natural killer cell function in malaria infection. In addition to its protective role in antiviral responses, MDA5 has been implicated in autoimmune and autoinflammatory diseases such as type 1 diabetes, systemic lupus erythematosus, and Aicardi-Goutieres syndrome
Purification Method:
Affinity purification
Gene ID:
64135
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from BxPC-3 cells, using IFIH1 Rabbit pAb (CAB13645) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
