The IDH3B Polyclonal Antibody (CAB13742) is a valuable tool for researchers studying the IDH3B protein, which plays a crucial role in cellular metabolism and energy production. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the IDH3B protein, researchers can detect and analyze this important enzyme in various cell types, making it an ideal choice for studies in biochemistry and cancer research.
IDH3B is a key enzyme in the tricarboxylic acid (TCA) cycle and is essential for producing energy in the form of ATP. Dysregulation of IDH3B has been linked to various diseases, including cancer and metabolic disorders. By understanding the function and regulation of IDH3B, researchers can uncover new potential therapeutic targets for these conditions and gain insights into the role of cellular metabolism in disease development.
Product Name:
IDH3B Rabbit Polyclonal Antibody
SKU:
CAB13742
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 35-170 of human IDH3B (NP_008830.2).
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Purification Method:
Affinity purification
Gene ID:
3420
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat heart, using IDH3B Rabbit pAb (CAB13742) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
