The IDH3A Polyclonal Antibody (CAB14650) is a valuable tool for researchers studying the IDH3A protein, an enzyme involved in the citric acid cycle and cellular metabolism. This antibody, raised in rabbits, is highly specific to human samples and has been rigorously tested for use in Western blot applications.IDH3A plays a crucial role in generating energy for cell growth and proliferation, making it a promising target for cancer research and metabolic studies. By detecting and quantifying IDH3A protein levels in various cell types, this antibody enables researchers to gain insights into the mechanisms underlying metabolic pathways and potential therapeutic interventions.
The IDH3A Polyclonal Antibody is essential for investigating the role of IDH3A in cellular metabolism, cancer progression, and metabolic disorders. Its high specificity and sensitivity make it an indispensable tool for advancing our understanding of IDH3A function and its implications for human health.
Product Name:
IDH3A Rabbit Polyclonal Antibody
SKU:
CAB14650
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 28-366 of human IDH3A (NP_005521.1).
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.
Purification Method:
Affinity purification
Gene ID:
3419
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using IDH3A Rabbit pAb (CAB14650) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
