The IDH2 Polyclonal Antibody (CAB7190) is a valuable tool for researchers studying IDH2, a key enzyme involved in cellular metabolism and implicated in various diseases, including cancer. This antibody, raised in rabbits, exhibits high reactivity with human samples and is specifically validated for use in Western blot applications.IDH2, also known as isocitrate dehydrogenase 2, plays a crucial role in the production of cellular energy and maintenance of cellular redox balance. Dysregulation of IDH2 has been linked to tumorigenesis and metabolic disorders, making it a promising target for therapeutic development.By binding to the IDH2 protein, this antibody enables accurate detection and analysis in various cell types, providing researchers with valuable insights into the role of IDH2 in disease pathways.
Its versatility and reliability make it an essential tool for studies in cancer biology, metabolism, and drug development.Understanding the function and regulation of IDH2 is essential for designing targeted therapies and interventions for diseases associated with IDH2 abnormalities. The IDH2 Polyclonal Antibody (CAB7190) is a reliable choice for researchers seeking to uncover the mechanisms underlying IDH2-related pathologies and develop novel treatments based on this important enzyme.
Product Name:
IDH2 Rabbit Polyclonal Antibody
SKU:
CAB7190
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 193-452 of human IDH2 (NP_002159.2).
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
3418
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using IDH2 Rabbit pAb (CAB7190) at 1:600 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
