The IDH1 Polyclonal Antibody (CAB2169) is a crucial tool for research related to Isocitrate Dehydrogenase 1 (IDH1), an enzyme involved in cellular metabolism and genetic mutations commonly found in various cancers. This polyclonal antibody, produced in rabbits, exhibits high specificity and sensitivity to human samples, enabling accurate detection and analysis of IDH1 in Western blot applications.IDH1 mutations have been implicated in the development and progression of several types of cancer, including gliomas, acute myeloid leukemia, and cholangiocarcinomas.
By targeting the IDH1 protein, researchers can gain valuable insights into the mechanisms driving tumorigenesis and potentially identify novel therapeutic targets for these malignancies.Furthermore, the IDH1 Polyclonal Antibody is an essential tool for studying the impact of IDH1 mutations on cellular metabolism, epigenetic regulation, and tumor microenvironment interactions. Its versatility and reliability make it an invaluable asset for investigations in oncology, precision medicine, and drug development aiming to target IDH1-mutated cancers.
Product Name:
IDH1 Rabbit Polyclonal Antibody
SKU:
CAB2169
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Monkey
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human IDH1 (NP_005887.2).
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3417
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using IDH1 antibody (CAB2169) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
