The IDH1 Polyclonal Antibody (CAB13245) is a specialized research tool designed for the study of IDH1, an important enzyme involved in cell metabolism and tumorigenesis. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the IDH1 protein, researchers can accurately detect and analyze its expression levels in various cell types, offering valuable insights for studies in cancer biology and metabolic disorders.IDH1, or isocitrate dehydrogenase 1, is a key enzyme in the tricarboxylic acid cycle and is frequently mutated in various cancers, including gliomas and acute myeloid leukemia.
Understanding the role and regulation of IDH1 is crucial for uncovering its implications in cancer progression and for developing targeted therapies. The IDH1 Polyclonal Antibody provides researchers with a reliable tool for investigating the function and dysregulation of IDH1, ultimately advancing our knowledge of cancer biology and identifying potential therapeutic strategies.
Product Name:
[KO Validated] IDH1 Rabbit Polyclonal Antibody
SKU:
CAB13245
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-414 of human IDH1 (NP_005887.2).
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26; H1
Positive Sample:
293T
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Peroxisome.
Calculated MW:
47kDa
Observed MW:
46kDa
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3417
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and IDH1 knockout (KO) 293T cells, using [KO Validated] IDH1 Rabbit pAb (CAB13245) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
