[KO Validated] IDH1 Rabbit Polyclonal Antibody (CAB13245)
- SKU:
- CAB13245
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
Product Name: | [KO Validated] IDH1 Rabbit Polyclonal Antibody |
SKU: | CAB13245 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-414 of human IDH1 (NP_005887.2). |
Sequence: | MSKK ISGG SVVE MQGD EMTR IIWE LIKE KLIF PYVE LDLH SYDL GIEN RDAT NDQV TKDA AEAI KKHN VGVK CATI TPDE KRVE EFKL KQMW KSPN GTIR NILG GTVF REAI ICKN IPRL VSGW VKPI IIGR HAYG DQYR ATDF VVPG PGKV EITY TPSD GTQK VTYL VHNF EEGG GVAM GMYN QDKS IEDF AHSS FQMA LSKG WPLY LSTK NTIL KKYD GRFK DIFQ EIYD KQYK SQFE AQKI WYEH RLID DMVA QAMK SEGG FIWA CKNY DGDV QSDS VAQG YGSL GMMT SVLV CPDG KTVE AEAA HGTV TRHY RMYQ KGQE TSTN PIAS IFAW TRGL AHRA KLDN NKEL AFFA NALE EVSI ETIE AGFM TKDL AACI KGLP NVQR SDYL NTFE FMDK LGEN LKIK LAQA KL |
Tested Applications: | WB IF/ICC IP ELISA |
Recommended Dilution: | WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
Synonyms: | IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26; H1 |
Positive Sample: | 293T |
Conjugate: | Unconjugated |
Cellular Localization: | Cytoplasm, Peroxisome. |
Calculated MW: | 47kDa |
Observed MW: | 46kDa |
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
Purification Method: | Affinity purification |
Gene ID: | 3417 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |