The Iba1 Rabbit Polyclonal Antibody (CAB1527) is a valuable tool for researchers studying Iba1, a marker for microglia and macrophages in the central nervous system. This antibody, raised in rabbits, exhibits high reactivity with human and mouse samples, making it suitable for use in various research applications such as immunofluorescence and immunohistochemistry.Iba1, also known as ionized calcium-binding adapter molecule 1, is a calcium-binding protein expressed in macrophages and microglia, playing a crucial role in the phagocytic activity and inflammatory response of these cells. Increased expression of Iba1 is often associated with neuroinflammation and neurodegenerative diseases, making it a promising target for research in areas such as Alzheimer's disease, Parkinson's disease, and multiple sclerosis.
By using the Iba1 Rabbit Polyclonal Antibody (CAB1527), researchers can effectively detect and visualize Iba1 expression in tissue samples, contributing to a better understanding of the role of microglia and macrophages in neurological disorders. Its specificity and sensitivity make it a reliable tool for studying the immune response in the central nervous system and developing potential therapeutic strategies for neuroinflammatory conditions.
Product Name:
Iba1 Rabbit Polyclonal Antibody
SKU:
CAB1527
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human AIF1/IBA1 (NP_001614.3).
This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain.
Purification Method:
Affinity purification
Gene ID:
199
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from THP-1 cells, using AIF1/IBA1 Rabbit pAb (CAB1527) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
